Sentence examples for medium samples was from inspiring English sources
Albumin concentration in the collected medium samples was analyzed using a competitive enzyme-linked immunosorbent assay [ELISA].
Albumin concentration of medium samples was analyzed by an enzyme-linked immunosorbent assay (ELISA), in triplicate, utilizing a polyclonal antibody to rat albumin (Cappel Laboratories, Aurora, OH).
Urea concentration of medium samples was determined via its specific colorimetric reaction with diacetyl monoxime using a commercially available assay kit (BUN Assay kit; Stanbio Laboratory, Boerne, TX) [29], [30].
Cell culture medium samples were collected every 2 h from 8 h post-induction.
Medium samples were taken by filtration of 2 ml of the culture broth through a 5-micron pore size nylon filter.
At the end of the experiment (on day 31), porous medium samples were destructively collected and analyzed for abundance of total and active bacterial cells, bacterial cell volume and concentration of polysaccharides and proteins.
The culture medium was replaced every 24h and medium samples were stored at −20°C for further analysis.
The culture medium was replaced every 24hr and medium samples were collected and stored −20°C until further analysis.
The paired transport medium samples were stored at 4°C for <4 days following collection prior to isolation in cell-culture.
Immediately after collection, the spent medium samples were cleaned of cell debris by a brief centrifugation step.
After removal of the medium samples were washed with 150 mM NaCl, 50 mM Tris, pH 7.4 and stored at −70°C.
