Sentence examples for medium same as from inspiring English sources

The mix (200 µl per plate) was plated on a 0.45-µm pore, 45-mm diameter nitrocellulose filter (Whatman) and placed on cocultivation medium (same as IM except that it contains 5 mM glucose instead of 10 mM glucose) in the presence of 200 µM AS and 40 mM MES (pH 5.3) (BioDee).

After 2 hours, the medium was replaced by serum free medium (same as above, without heat-inactivated FCS) for overnight incubation prior to stimulation.

After a 1-h adsorption, 2 mL of nutrient medium (same as above but 5% FBS) was added to tissue culture wells, which were incubated as above for up to 7 days.

Subsequently, one volume unit of starvation medium (same as Spizizen salts minimal medium, but without casamino acids and L-tryptophan) was supplemented and growth was continued for two more hours.

The explants that had an outgrowth of cells with fibroblastic (elongated) morphology were selected and isolated with clonal rings, and the outgrowth of cells was trypsinized and subsequently cultured in fibroblast routine culture medium (same as above).

At 85% confluence, the growth medium was changed for infection medium (same as the growth medium without FBS) and cells were allowed to grow for another 24 hr period before infection with bacterial pathogens.

Cells were harvested at room temperature at 3000 ×g for 5 min and suspended in 50 mL of YPTM medium (same composition as YPTG except that glycerol was substituted with 1% methanol).

Bacterial cells were grown at 37°C with shaking at 220 rpm in LB broth (Lennox) medium or minimal medium (the same as M9 but without NH4Cl; 12.8 g Na2HPO4 7H2O, 3 g KH2PO4, 0.5 g NaCl per liter).

The test was performed by adding known amounts of the 9 standards at low (80% of the known amounts), medium (the same as the known amounts) and high (120% of the known amounts) levels.

The composition of the latter medium was the same as the growth medium but with reduced amounts of glucose (10 g l−1) and yeast extract (0.25 g l−1) and the nitrogen source was NH4NO3 (0.3 g l−1).

The differentiation medium was the same as the hESC medium except that the KOSR was replaced with 20% non-heat-inactivated FCS.