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The results showed that sonication frequency of 574 kHz generate maximum cavitation in the medium resulting higher rate of disintegration as compared to other frequencies tested.
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One the other hand, it can facilitate the monomer diffusion (i.e., increased solubility of monomer in the medium), resulting in higher number of nuclei.
However, Aurass et al. [ 85] demonstrated that a bdhA-patD mutant strain of Lp is defective in breaking down PHB accumulated during growth in a rich medium, resulting in a higher level of cellular PHB than the wild-type.
Saturated operation meant that the maximum possible power was extracted from the lasing medium, resulting in the high peak energy of 0.3 mJ.
Our results show that inclusion of an amino acid in the growth medium resulted in higher (Table 1) total biomass accumulation and that biomass partitioning to roots in all cases was significantly enhanced (Fig. 1).
Consistent with the result that intracellular ROS mediated plasma induced DNA damage, we found that longer incubation in the original 100 µl of medium, in which cells were treated (before dilution into 2 ml of medium), resulted in higher levels of γ-H2AX (Figure 5A).
In this model, the penalized porous region acts as a high impedance medium, resulting in negligible wave transmissions.
We showed the use of this medium resulted in significantly higher yields of rSAK2 compared to "classical" unmodified SYN6 growth medium, which has often been used for H. polymorpha fermentations [ 11].
Statistical analysis of the experimental data using the response surface methodology showed that a glycerol-to-ornithine molar ratio of approximately 40 1 in the feed medium resulted in the highest CA concentration when fermentation was stopped.
Semi-batch operations with 50% replacement of culture medium resulted in the highest CO2 fixation rate (1546.7 mg L−1 d−1), carbohydrate productivity (467.6 mg L−1 d−1), and bioethanol yield (0.202 g/g biomass).
In the present study, the use of 5%% (v/v) of B. marisflavi NA8 in POME medium resulted in the highest flocculating activity.
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