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Through statistical optimization method, 32-fold increase in κ-carrageenase production was achieved as compared to medium reported in the literature.
The preculture was performed in 1 L shakeflasks filled with 100 mL of medium, reported in the previous section, and grown on a rotary shaker at 200 rpm and 37 °C for 12 14 h.
The current results show that the cell-based H5N1 viral antigens produced from SF medium could induce strong and efficacious immune responses similar to those obtained from the H5N1 virus produced from the SC medium reported in our previous study [4].
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Addition of surfactants and polyhydroxy alcohols in production medium reported to increase amylase secretion [ 32].
The magnetite nanoparticles were obtained by co-precipitation of iron II) and (III) salts in a basic medium, reported by [12].
Both MFC-U and MFC-C were fed with a digestate-based medium as reported in materials and methods.
The coconut water sucrose medium as reported in the work of Verschuren et al. was used in this study with a slight modification [ 19].
Rather the responses to specific growth factors or cocktails of factors (such as fibroblast conditioned medium) are reported in such a way that these conditions likely apply to all cancer cells, or at least a particular subtype of cancer.
Taurine and hypotaurine levels in cells and medium are reported in Figs. 2 and 3. Taurine levels ranged from 10to1717 nmol/mg protein in hepatocytes from Cdo1+ / + mice (Fig. 2), whereas hypotaurine levels ranged from 4 to 8 nmol/mg protein in hepatocytes from wild-type mice (Fig. 3).
For best depiction of delayed enhancement, we chose a delay time of 13 to 17 min after contrast medium application, as reported in the literature.
Some influenza virus production in MDCK cells grown on microcarriers in SF medium have been reported in the literature [16], [20].
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