Sentence examples for medium replenishment from inspiring English sources

Removal of mono-chlorobenzene (m-CB) vapors from air streams in a biotrickling filter unit was studied under various operating conditions concerning inlet m-CB concentration (Cgi), air residence time, liquid recirculation rate (Q), pH, and frequency of medium replenishment.

Subsequently, the cells were maintained in KO with medium replenishment carried out on every other day or depending on the cellular demand.

The density higher than 100 K at times led to pronounced cell death because of cell overgrowth and hence required frequent medium replenishment.

The cells were maintained for 15 18 days with medium replenishment every 2 to 3 days.

Then, 72 h following compound addition, 50% medium replenishment was performed as described above.

Medium replenishment and imaging was performed as shown on the schematic protocol in Figure 6a.

Finally the reuse of spent medium with proper replenishment of nutrients (nitrate, phosphate, chromium, selenium and copper) caused an unexpected 30% drop in the growth rate and did not seem to provide an attractive response as the total cost per 0.5 kg dry cell was only saved by 8 US$ a year.

When treatment was indicated, the agonist and antagonists were added after 8 to 10 days of incubation at 10 nM for Am580, 200 nM for at RA, and 10-fold excess of Ro41-5253 Rantagonistnist, 100 nM or 2 μM, respectively, and the mammospheres were incubated for approximately eight days with partial medium and drug replenishment every two days.

Then, scaffolds were incubated in DMEM for 21 days with regular replenishment of medium every alternate day.

First, most static culture methods do not allow for the constant replenishment of medium and important nutrients.

The plates were incubated for 14 days in a 5% CO2 incubator at 37 °C, with replenishment of medium every 3 days.