Sentence examples for medium replenished with from inspiring English sources

However, the Authors showed no effects of the inhibitor on SMARCB1-wild-type RD cells that were cultured in medium replenished with the drug on day 4 [ 50].

Then, the cells were treated with control or 1 mM NMDA in normal medium, rADI-pretreated medium, 1 mM NMDA in rADI-pretreated medium, or 1 mM NMDA in rADI-pretreated medium replenished with 0.8 mM L-arginine for 24 h.

Figure 3 shows the results of the rhodamine 123 fluorescence intensity measured in cells under the following conditions: control without any treatment, 1 mM NMDA in normal medium, absence of NMDA in rADI pretreated medium, 1 mM NMDA in rADI pretreated medium, and 1 mM NMDA in rADI-pretreated medium replenished with 0.8 mM arginine for 24 h.

On day 4, EBs were manually removed and collected in non-adherent plates and maintained in the respective compound/DMSO/untreated medium; the culture medium was replenished with fresh medium every other day.

After 2 days, half of the medium was replenished with medium with/without Aza (5 μM).

After 2 d, half of the medium was replenished with medium with/without 5-Aza-dC (5 μM).

The cells were then washed with growth medium to remove the detached cells, and the medium was replenished with fresh medium (Das et al, 2013).

Following the incubation period, inoculation medium was discarded and the medium was replenished with 3 ml of fresh medium.

Cells were incubated for 48h at 37°C, and the medium was replenished with fresh medium containing cisplatin (10μM).

After 5 7 days, one-half of the medium was replenished with fresh medium containing 40 ng ml−1 rIL-7 and 50 IU ml−1 rIL-2.

The culture medium was replenished with fresh medium maintaining the glucose concentration either with IL-1β (PeproTech EC Ltd, London, UK) alone or with IL-1β + TNF-α (PeproTech EC Ltd., London, UK) + IFN-γ (PeproTech EC Ltd, London, UK).