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After 24 h, the culture supernatant was removed, and the medium replaced with BMMY.
The supernatant was removed and the medium replaced with BMMY to induce expression.
Secondly, whereas the initial method employed a wash for the removal of EB, the medium replaced with EB free-saline, and the efflux of EB was then followed, in the current method after the accumulation phase, glucose-saline of matching pH is added and, in accordance to the criteria governing the source of energy driving the pump, efflux of EB is followed.
The four levels of financial sustainability were the following: (1) None: AD syringes not replaced or partially replaced; (2) Low: replaced but fully donor dependent; (3) Medium: replaced with mixed government and donor funding; and (4) High: replaced with full domestic funding.
On day 4, the cells were washed and the medium replaced with serum-free medium.
Cells were incubated for 24 h and the medium replaced with OPTI-MEM-reduced serum medium (Invitrogen).
Similar(36)
After 24 h the normal medium was replaced with medium containing Vit-D (10-8 M).
After seeding, the growth medium was replaced with medium containing indicated concentration of TSA.
Prior to treatment, the culture medium was replaced with medium supplemented with 2% fetal bovine serum.
To chase, radioactive medium was replaced with medium supplemented with 1 mM methionine/cysteine (Sigma).
Four hours later growth medium was replaced with medium containing either 10% or 0.5% FCS.
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