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The relative mRNA expression of each point in time was compared to the mRNA expression of C. albicans grown on YPD (rich) medium (referred to as common reference).
To analyze gene transcription and to measure ustiloxin B production, strains were cultured in V8-juice liquid medium referred to hereafter as V8 medium [20%% (v/v) V8 juice (Campbell's, Camden, NJ, USA) with 0.3 % CaCO3] at 30 °C on a rotary shaker at 160 rpm.
Earlier we have shown that normal human adult epidermal melanocytes can grow in a medium (referred to as Mel-mix) that lacked the above listed mitogens.
The mononuclear cell layer was isolated by density gradient using UNI-SEP maxi tubes (Novamed, Israel) centrifuged at 1000 g for 20 minutes, and resuspended in 6 volumes of medium (referred from here onwards as FBS).
For each sample, two subpopulations were isolated: sperm that migrated to the medium (referred here as migrated sperm); sperm that did not migrate (referred herein as non-migrated sperm).
The labelled sections were rinsed for 10 min in two changes of ice-cold PBS and then mounted in the Mowiol mounting medium referred to earlier.
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understand that a medium refers to an environment.
Prior knowledge: Prior to this lesson, students should be able to state that heat is a form of energy understand that a medium refers to an environment.
Conditioning of cell culture medium refers to exposure of the NPs in an environment that the cells have been cultured for a certain amount of time, thereby containing all the by-products of cellular metabolic activity.
We then performed a cytokine array to search for cytokines and/or chemokines released in the culture medium (hereafter referred as conditioned medium, i.e. medium of cells treated with 50 nM doxorubicin for 2 days).
The two conditioned medium are referred as frontal conditioned medium (FCM) and parietal conditioned medium (PCM).
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