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The second form (GenBank FJ010170) includes exons 11-12-14 and encodes a protein with a medium reading frame terminated by an alternative stop codon in the beginning of exon 14 (Fig. 2).
All assays of TCF7L2 with significantly increased expression in subcutaneous compared to omental adipose ("ex12-13", "ex13-13a" and "ex12-14") detect splicing forms that encode truncated TCF7L2 protein with short or medium reading frames and include an alternative exon 12 (Figure 2).
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We generated both medium reads (∼250 bases) and short reads (∼150 bases) using the Illumina MiSeq and HiSeq2500 sequencers, respectively.
Several assembly programs dedicated to short or medium reads were used to generate contigs: Velvet (www.ebi.ac.uk/~zerbino/velvet), SOAPdenovo (http://soap.genomics.org.cn), and CLC Genomics Workbench (www.clcbio.com) (J. Cheval et al., unpub. data).
Several algorithms have been developed to assemble short (<100 bp) and medium reads (e.g. the Roche 454 FLX Titanium platform with average read length of ~400 bp was released in October 2008), including efforts by the commercial providers of sequencing technologies (e.g. the Newbler assembler from Roche).
He uses these objects as communicative mediums," reads the artist's bio on his website.
Some predicted that electronic books would become the main medium for reading by 2015.
State censorship and strict rules imposed on satirical publications or TV and radio programmes mean that mobile phones are perhaps the most popular medium for reading jokes.
But for its reputation to be assured as a medium for reading pleasure, the desire to go public straight away must be resisted.
Yet as the Web continues to emerge as a medium for reading, it often grapples with issues of seriousness and taste.
Twitter would bring mature advertising technology to Flipboard's reader app, offering the chance for the product to start bringing in real revenue; while Flipboard could offer an easier, more appealing product for new users who want to treat Twitter as a one-way medium for reading content shared by others.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com