Currents generated in these reactions may be linearly dependent on the concentration of the electroactive molecules in the extracellular medium, enabling quantification of these compounds.
Although the present bioethanol fermentation was performed using a synthetic medium, the impact of using an actual lignocellulosic hydrolysate or other complex fermentation medium on the quantification of ethanol in the headspace would be anticipated to be minimal.
We used selective lactose fermentation phenomena of enteric bacteria on MacConkey medium for the quantification of bacteria cultural characteristics.
Attempts to optimize pollen germination in an artificial medium for precise quantification of pollen viability failed as results were inconsistent (data not shown).
The MFIh-CO2 was determined by addition of C-labeled sodium bicarbonate into the culture medium, followed by quantification of the isotropic-labeled and unlabeled forms of one intracellular metabolite by liquid chromatography mass spectrometry/mass spectrometry (LC-MS/MS).
(A ) Maximum ΔF/F of each individual wild-type animal's AWCON, AWA, ASEL or AWB neuron response to medium BZ. (B ) Quantification of the time to maximum ΔF/F following stimulus change for each wild-type AWCON, AWA, ASEL or AWB neuron response to medium BZ in seconds.
Isolated mitochondria were resuspended in 100 μl of homogenisation medium A. After quantification of protein with Bradford method (Biorad), the sample was incubated for 5 min at 4°C with Digitonin (Fluka) at a final concentration of 0.18 mg for mg of protein.
Supernatants of cell medium were frozen for quantification of leptin by ELISA.
After 1 h, medium was recovered for quantification of insulin and serotonin release using commercial ELISA kits (LDN, Nordhorn, Germany; Mercodia, Uppsala, Sweden, respectively).
Because all experiments with warm background involved phantoms with uniform distribution of attenuating medium, the quantification accuracy of both scatter correction methods was very similar for all three segmentation approaches.
