Sentence examples for medium prepared from from inspiring English sources

Human neuroblastoma cells SH-SY5Y was purchased from the American Type Culture Collection (ATCC no. CRL-2266) and cultured in complete medium prepared from Dulbecco's Modified Eagle's Medium (Gibco, UK) supplemented with 10% fetal bovine serum (Gibco, UK).

In this study, we established a model in which primary beta-cells were treated with conditioned medium prepared from human primary myotubes obtained from vastus lateralis biopsies.

Our results showed that MADM microcarriers retained the ultrastructure of the acellular dermal matrix, had good biocompatibility, and supported human fibroblast expansion either as a direct culture substrate or through culturing cells in conditioned medium prepared from them.

Overnight culture of the strains carrying GFP reporter constructs were diluted 100 folds and grown for 6 h in LB containing 50% (v/v) spent medium prepared from recombinant E. coli cultures expressing cloned CAI-1 or AI-2, and from control E. coli cultures carrying the empty vector pTAC.

To study the effect of autoinducers on the phage bacterial growth kinetics, the medium was supplemented with 50% (v/v) spent medium prepared from recombinant E. coli over-expressing or not expressing the V. cholerae autoinducers CAI-1, and AI-2.

We observed that the incubation of MSC with a conditioned medium prepared from chemically damaged but not undamaged muscle resulted in a time-dependent change from fibroblast-like into elongated multinucleated cells, a transient increase in the number of MyoD positive cells, and the subsequent onset of myogenin, α-actinin, and myosin heavy chain expression.

All copepods were starved for 24 hours in K-medium prepared from filtered seawater (0.2 µm; salinity ∼33‰) at 15°C in darkness before each experiment.

The conditioned medium was prepared from medium incubated for 48 hrs in proliferating precursor cells.

Briefly, Farrel's medium was prepared from Brucella medium base (Oxoid, UK), sterilized at 121°C for 15 minutes, and supplemented with Brucella selective supplement (Oxoid, UK) according to the manufacturer's instructions.

The culture medium was prepared from 90% Dulbecco's modified eagle medium-nutrient mixture F-12 (DMEM-F12), 10% heat-denatured fetal calf serum (FCS), 6 g/L D-glucose, 2 nM glutamine, 25  μg/mL gentamycin, and 50 ng/mL IGF-I (all purchased from Biological Industries, Israel).

Conditioned medium was prepared from A. machipongonensis grown in seawater complete medium (750 mL) at 30°C for 2 days.