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Human neuroblastoma cells SH-SY5Y was purchased from the American Type Culture Collection (ATCC no. CRL-2266) and cultured in complete medium prepared from Dulbecco's Modified Eagle's Medium (Gibco, UK) supplemented with 10% fetal bovine serum (Gibco, UK).
In this study, we established a model in which primary beta-cells were treated with conditioned medium prepared from human primary myotubes obtained from vastus lateralis biopsies.
The phosphate medium prepared PAni was a strong reducing agent and radical scavenger.
Our results showed that MADM microcarriers retained the ultrastructure of the acellular dermal matrix, had good biocompatibility, and supported human fibroblast expansion either as a direct culture substrate or through culturing cells in conditioned medium prepared from them.
For this reason, this paper proposes a first test to assess the capacity of ten different geotextiles by immersing them in a culture medium prepared to favour the growth of a microbial community.
In order to validate the adequacy of the model, the confirmation experiment was carried out using medium prepared according to the RSM results.
On the day of the experiment, cells were washed twice with ice-cold internalization medium prepared with F-12-K nutrient mixture supplemented by 1% (v/v) fetal bovine serum.
Microorganisms capable of utilizing vegetable tissues for multiplication in soil were isolated, cultivated in liquid medium prepared from the same vegetable tissues, and tested for ability to activate resistance in pepper leaves against Phytophthora blight caused by Phytophthora capsici.
Standard Pro99 medium prepared with filtered, autoclaved seawater was found to contain approximately 0.2±0.02 µM HOOH.
Strains were grown on YPD medium (1% yeast extract, 2% Bacto-peptone, and 2% glucose) or synthetic complete medium prepared as described [23].
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All copepods were starved for 24 hours in K-medium prepared from filtered seawater (0.2 µm; salinity ∼33‰) at 15°C in darkness before each experiment.
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