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The total number of cells positively stained by each marker was determined by counting multiple grids randomly placed on tissue sections at medium power field magnification (40×).
In short, collagen III expression, myofibroblasts, ED1-positive macrophages and CD3-positive T cells were evaluated in 30 (high or medium power field) cortical interstitial areas of each kidney in a blinded fashion.
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For each transwell, the number of migrated cells in 10 medium power fields (× 20) was counted.
Nuclear bags and fiber splittings were counted in medium power fields corresponding to a 200-fold magnification.
The cells were counted in at least 10 medium power fields using a calibrated graticule, then the number of cells per mm−2 was calculated.
Five medium power fields per section were viewed and tumours were given a semi-quantitative score of 0, <50, and ⩾50% CD133+ tumour glands.
The correlation between expression of the proteins was determined by counting the positive cells in at least four medium power fields of serial sections, calculating and presenting as means ± SD.
A total of 4 adjacent medium power microscopic fields were analyzed at the power of ×20.
But their meeting enables to create a new size in wind energy: the medium power wind energy, the field of action of VERGNET.
The Netherlands, as a 'pocket-sized medium power', is an interesting case study.
At high power field, a polymorphous population of small- to medium-sized lymphocytes, plasma cells, plasmacytoid cells, as well as large, basophilic transformed lymphocytes and immunoblasts diffusely infiltrated the paracortical area.
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