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To address this issue we designed a plasmid swap experiment assuming that the pribH1Km plasmid that is harbored by the ribH1-ribH2 strain is essential for the growth in TSB medium (poor in riboflavin) (Figure 2A).
Thus, we inhibited CCM3 in an immortal cell line derived from retinal epithelial cells (hTERT-RPE1) (Fig. S5A, Supporting information) and then induced autophagy by incubating them in Hank's Buffered Saline Solution (HBSS), a medium poor in amino acids.
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In general, this medium is poor in organic carbon, which effectively controlled fungal and eubacterial growth and thus helping in the isolation of the slower growing actinomycetes [ 22].
DMEM-Advanced was also trialled as a base medium for the differentiation medium, but survival of both neurones and oligodendrocytes was poor in this medium (compare Fig. 4A and B with Fig. 4C and D).
Reproducibility decreases across the medium stratum, and is consistently poor in the low stratum.
Effective poverty interventions should be targeted at most poor, not the medium-poor.
In Sweden and Poland, the reliability of the certification body is good (indicator 34), while it is poor in Estonia and Latvia, and medium in Lithuania (Table 9).
We examined the relationship between the age of the mother at diagnosis and the concordance in prognosis (good, medium, or poor) and assessed the trend in relation to age.
NK cells cultured in medium exhibited poor cytolytic activity against Raji lymphoma cells that are resistant to NK-mediated killing (Fig. 4a).
The only nutrients more abundant in minimal medium than in poor medium are phosphate and ammonium salts (M2 salts), and FeSO4.
We found that a persons shedder status – good, medium or poor – is of consequence in order to deposit enough DNA for a full profile.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com