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The medium plates were incubated at 30°C for seven days.
The YPD agar medium plates were incubated at 30 °C for 48 h and nutrient agar plates were incubated at 37 °C for 24 h.
A well was developed in the sterile Petri dish with medium plates were prepared with the help of cork borer (6 mm in diameter) and (50 μL) solution of each concentration was poured into the wells.
Medium plates were incubated at 30°C.
Semi-solid medium plates were scanned on a CellCelector™ (Aviso).
Solid medium plates were inoculated with 1 × 10 spores.
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The aspect of the colony on rich medium plate was slightly different (Fig. 3).
When needed, medium and plates were supplemented with ampicillin (50 μg/mL), kanamycin (50 μg/mL) or chloramphenicol (35 μg/mL).
After incubation at 37 °C for 2 days, the colonies of strain WZN-1 on LB medium agar plates were approximately 1 2 mm in diameter.
EMM medium and plates were prepared using EMM broth, powder (MP Biomedicals).
After removal of uptake medium, the plates were immediately placed on ice and cells were lysed in ice-cold lysis buffer.
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