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For spreading analysis, cells were trypsinized, resuspended in complete medium, plated on sterile coverslips coated with an adhesive substrate, and allowed to spread for various times.
The pellet was re-suspended in 250 μl 2xYT medium, plated on a 15 cm 2xYT-GA agar plate and incubated at 37 °C over night.
After centrifugation, cells were resuspended in 4 ml complete growth medium, plated on a T25 plate (Greiner Bio-One, Frickenhausen, Germany) and grown until confluent.
15 After 24 h, oesophageal cells from primary cultures were sorted in 5% FBS DMEM in 2 mL Eppendorf tubes, resuspended in FAD medium, plated on the collagen rafts and incubated at 37°C, 5% CO2.
After 2 additional washes with cold DMEM, the pellet was resuspended in endothelial growth medium, plated on 0.5% gelatin-coated plastic ware and incubated at 37°C in a 5% CO2-air atmosphere.
For analysis of cultivable bacterial species guts from workers of three colonies were homogenized in 50 μl Luria Bertani (LB) medium, plated on LB agar plates and incubated overnight to several days at 30°C and 37°C.
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One isolated colony was grown in 300 μl YPD medium overnight, plated on YPD medium and incubated at 30°C for 4 5 days.
The multimodal measurements are here illustrated by measuring live mouse embryonic fibroblasts cells (MEF) [18], cultured in Dulbecco's modified Eagle medium, and plated on quartz-bottom dishes one day prior experiment.
1.5×10 cells were resuspended in serum-free medium and plated on to the top of each chamber insert and serum-free medium was added to the bottom chamber.
E. coli strains DH5α and BL21 were incubated in Luria-Bertani (LB) medium or plated on LB agar.
E. coli DH5α was cultured in Luria Broth (LB) liquid medium or plated on LB agar.
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