Sentence examples for medium perfusion and from inspiring English sources

Endothelial [Ca2+]i reached a mean peak value of 324±35 nM in the first 2 3 minutes of B31-A3 spent medium perfusion, and fell to a sustained level (160±22 nM) after 10 min. When each vessel was recannulated and perfused with albumin-Ringer solution, [Ca2+]i returned to the baseline level, 86±4 nM (Fig. 5A).

The system features two separated channels for medium perfusion and oxygenation.

The system mainly integrates the functions of continuous culture medium perfusion and the generation of pneumatically-driven compressive stimulation in a high-throughput micro cell culture system.

The PDMS chip consists of two layers, the upper containing the flow channels for medium perfusion and the lower raising these channels 120 μm above the culture plane.

Thus, they proposed the design of a bioreactor to deliver simultaneous culture medium perfusion and electrical stimulation during the culture of engineered cardiac constructs free of external fixation.

To tackle the above technical issues, more recently, perfusion-based microscale bioreactor systems were actively proposed for various cell-based assays [ 6, 8– 10] by which a stable and well-defined culture condition can be achieved due to the continuous medium perfusion format and miniaturized cell culture scale [ 6, 8].

The objective of the study was to investigate the combined effects of three sets of regulatory factors: cell pre-differentiation, soluble factors and medium perfusion on spatial control of human mesenchymal stem cell (hMSC) differentiation into cells forming the cartilaginous and bone regions in engineered osteochondral constructs.

Each vessel was then perfused with B31-A3 spent medium; the measured endothelial [Ca2+]i reached a mean peak value of 301±15 nM within 3 min of B31-A3 spent medium perfusion (Fig. 5B and Fig. 5C, N = 4).

These include increasing the external mass transport by convective mixing of culture medium around the constructs, increasing the internal mass transfer by medium perfusion through cultured tissues in vitro, and vascularization of the cultured tissues in vivo.

One mM H2O2, administered 20 minutes prior to and during hypoosmotic medium perfusion, did not affect basal levels of glutamate or aspartate but significantly enhanced the swelling-evoked release of both excitatory amino acids (Fig. 8a,b).

After ATDC5 cells transfected with PxRe reporter gene grew to confluence in the maintenance medium, perfusion cultures were performed by perfusing the chondrogenic medium including D-luciferin at a flow rate of 1.0 mL/h by a peristaltic pump.