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Exact(9)
Cyclohexane-tolerant mutants that formed colonies on the LBGMg agar medium overlaid with cyclohexane were isolated from strain JA300.
Spontaneous cyclohexane-tolerant mutants from strain JA300 appeared on the LBGMg agar medium overlaid with cyclohexane at a frequency of 1.6 × 10-6.
A 100-μl culture of overnight-grown E. coli strain was inoculated to 10 ml of fresh LBGMg liquid medium overlaid with an organic solvent.
In the present study, to efficiently isolate mutants with high-level organic solvent tolerance, the parent strain JA300 was precultured in the LBGMg liquid medium overlaid with cyclohexane.
First, 100 μl of the overnight culture of strain JA300 grown in LBGMg medium was inoculated into 10 ml of fresh LBGMg medium overlaid with 2 ml cyclohexane.
Five days later, when most cells were infected, a wet mount was prepared (~2 × 106 cells in 15 μL medium overlaid with a cover slip) and microscopically examined by epifluorescence.
Similar(51)
The solidified medium was overlaid with liquid MM (Figure 1A).
Medium was overlaid with heavy mineral oil to prevent oxygen back diffusion.
Culture medium was overlaid with pre-equilibrated mineral oil (Sigma-Aldrich; M8410) to prevent evaporation.
The medium was overlaid with a layer of nylon mesh and 4-d-old Arabidopsis seedlings grew on the nylon mesh to facilitate collection of root samples.
All strains were maintained on solid nematode growth medium (NGM) overlaid with E. coli (OP50), at 20 °C except for upshift of strains CL4176 and CL2355 to 25 °C during the L3 L4 transition to induce Aβ1 42.
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