The renal fibroblasts were treated with either medium or various stimuli; Pam3CysSK4, ultrapure LPS (both Invivogen), CpG-ODN 1668 (TIB Molbiol, Berlin, Germany), and TNF-α (ImmunTools, Firesoythe, Germany.
Parasites at 8 × 106 cells/mL were incubated in culture medium or various buffers for 2 hours at 26°C either alone or in the presence of 10 μM halofantrine.
PMN (10/mL) were incubated at 37°C with medium (control) or various concentrations of levosimendan (10 to 1000 ng/mL) for 30 minutes.
After overnight incubation, cells were incubated in drug-free medium, or medium containing various concentrations of 17-AAG, or 17-AAG in combination with cisplatin for 48 h at 37°C.
After adhesion, cells were cultured in standard culture medium or treated with various concentrations of curcumin CD complexes and/or gemcitabine (1 5 10 ng ml−1) or with vehicle alone as control for 24 and 48 h.
Broad indicators, such as indication of high, medium, or low for various factors in the assessment, might be used to communicate complicated and disparate data related to exposure (e.g., emission inventories, environmental concentrations) and toxicity [such as toxicity indicators displayed using geographic information systems (GIS)].
J774.A1 cells were plated at a density of 1.5 × 10 cells/well in a final volume of 200 μl in 96-well flat-bottom tissue culture plates and incubated in medium alone or medium containing various concentrations of polysaccharide fractions or lipopolysaccharide (LPS) from Escherichia coli K-235 (Sigma Chemical Co., St . Louis MO) as a positive control.
NB cells were exposed to control medium or to medium containing various concentrations of TNF-α, and cell survival was measured by MTT assay 24 h later.
Overnight cultures were diluted to a starting density of A600 = 0.5 and were spotted in 10-fold serial dilutions onto YPD plates or medium containing various concentrations of drugs as indicated.
Cultures were grown in chemically defined medium containing either ammonium sulphate or various amino acids as sole nitrogen source.
PMN (5 × 10/mL) were incubated at 37°C (5% CO2 atmosphere) with medium (i.e. control) or various concentrations of levosimendan (Simdax™, Abbott, Vienna, Austria) ranging from 10 to 1000 ng/mL and then brought to a final volume of 200 μl/well (96-well plate) containing 10 μM 2',7'dichlorodihydrofluoresceindiacetate (H2DCFDA; Invitrogen, Carlsbad, CA, USA) in HBSS.
