The E. coli LMC500 (29, 30) and SF100 (31) wild-type strains were grown in minimal medium GB1 or rich medium TY as described (32).
Similarly, Z. mobilis 2032 was inoculated into 5 mL ZRMG medium (or RM, rich medium) containing 20 g/L glucose; 10 g/L yeast extract; and 2 g/L KH2PO4 [ 66], with 12.5 g/L each of tetracycline (Tc) and chloramphenicol (Cm), and grown under aerobic conditions at 30 °C.
Growth curves at shake flask-scale of the ex-conjugants S. coelicolor ΔvanRS, S. lividans and S. venezuelae carrying pIJ86ΩC-His6 -vanY n or pIJ86ΩN-His6- vanY n were compared with control strains containing the empty vector in two different cultivation conditions, i.e. by using the limpid YEME medium or the rich and complex BTSB medium.
A formation of flocs with the subsequent rapid sedimentation from the medium was observed regardless of the type of medium used (defined or rich).
Forest income was more important for poor households (47.3%) than for medium (30.5%) or rich (20.2%) households.
Each predicted biosensor strain is expected to grow in a spent medium (supplemented with either glucose minimal or rich medium) in the presence of its sensed chemical, and not grow in its absence, regardless of which additional chemicals were potentially secreted by the producer strain.
Recombinant cells were cultured in seed medium (20 g/L yeast extract) or rich growth medium (20.3 g/L yeast extract (BioSpringer, Milwaukee, WI, USA) 10.1 g/L sodium sulfate anhydrous (JT baker, Center Valley, PA, USA) and 7 g/L K2HPO4 (JT baker, Center Valley, PA, USA) both supplemented with 50 μg/mL kanamycin (Sigma, St . Louis MO, USA) for expression of recombinant ATH35L proteins at 30 °C.
The chromogenic β-galactosidase substrate 5-bromo-4-chloro-3-indolyl-β d-galactopyranoside (X-Gal) was used at a concentration of 25 μg/ml (minimal medium) or 40 μg/ml (rich medium).
Cells were grown in rich medium or in minimal medium supplemented or not with polygalacturonic acid (PGA), arabinose, glucose, maltose, sucrose, xylose or xylan.
All media were standard, either YPD for glucose rich medium, or synthetic complete media when the presence of a plasmid was selected for.
