Sentence examples for medium or recombinant from inspiring English sources

Treatment with Dkk1-conditioned medium or recombinant Dkk1 protein stabilized LRP6 with a prolonged half-life and induces LRP6 accumulation both at the cell surface and in endosomes.

Treatment of OECs with reactive astrocyte-conditioned medium or recombinant TNF-α induced a significant increase in the amount of phosphorylated ERK1/2, which was greatly attenuated by pretreatment of cells with PD98059, a selective MAPK inhibitor (Fig. 5B, C).

As shown in Fig. 5A and B, stimulation of OECs with reactive astrocyte-conditioned medium or recombinant TNF-α protein resulted in prominent activation of extracellular signal-regulated kinase 1/2 (ERK1/2).

In present study, we found that the amount of active ERK1/2 was significantly increased in OECs treated with reactive astrocyte-conditioned medium or recombinant TNF-α, which suggested that TNF-α binding to TNFR1 led to activation of ERK1/2 in OECs.

The cells were then treated with Sema3C-conditioned medium or recombinant protein for 30 min and changes of cell morphology were analyzed.

A volume of 100 µl of control medium or recombinant endostatin at 50, 500 and 500 ng/ml (n = 4) or angiogenin at 10, 100 and 1000 ng/ml (n = 4), with or without 10 ng/ml EGF, was added in serum-free media and spheroids were visualized after 24 h incubation using an Olympus 1X70 inverted microscope.

Active TGFβ was determined after overnight incubation of the reporter cells with conditioned medium or with recombinant human TGFβ1 standards.

The plate was blocked in 1 μg/mL bovine serum albumin (BSA) (Sigma-Aldrich, St Louis, MO, USA) in phosphate-buffered saline (PBS) (4 hours) prior to incubation with 200 μL of conditioned medium or serially diluted recombinant elafin (Calbiochem, Merck Millipore, Billerica, MA, USA) as a control.

Pretreating OECs with this pharmacological inhibitor blocked the activation of ERK1/2 and greatly attenuated the migration of these cells induced by reactive astrocyte-conditioned medium, glial scar tissue, or recombinant TNF-α.

To induce angiogenesis, 33 μl of either the culture medium to be tested or recombinant human basic Fibroblast Growth Factor (bFGF) (R&D Systems) (positive control) were next placed inside the rings on successive days for 3 days.

Differentiated human adipocytes were incubated with either RPMI-1640 (control), MC medium, MC medium neutralized by an IL-1β antibody (R&D), MC medium neutralized by an IL-1β antibody and a TNFα antibody (R&D), mouse IgG (Sigma), or MC medium with recombinant IL-1RA (Sigma) for 24 h.