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Bacterial cultivation medium was either Luria-Bertani (LB) medium or minimal salt basal medium (MSB) (Kongpol et al. 2008).
Burkholderia sp. SJ98 was grown aerobically in Luria-Bertani (LB) medium or minimal medium (MM) prepared (Samanta el al. 2000) at 30°C.
S. pombe strains (Table S1) were grown in yeast extract medium or minimal medium with appropriate supplements [29].
Yeast was grown at 30°C in either standard YPD medium or minimal medium lacking leucine (leu) to select for appropriate plasmids.
The indices were transformed for each sampling event in a weighting factor (wsk) used to classify their value for monitoring as optimal, medium or minimal.
In contrast, gas2Δ mutants and gas5Δ mutants, and even the double gas2Δ gas5Δ mutant, were viable at all temperatures and showed no apparent growth defect in rich medium or minimal medium (data not shown).
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Cells were grown in rich medium (YPD), complete medium (CM) or minimal medium (MM) [46].
All geneticin-resistant colonies were plated in duplicate onto either minimal methanol medium (MM) or minimal dextrose medium (MD) plates to characterize the methanol-utilizing phenotype.
The three panels represent serial dilutions (1 10) of mc2155 and mc251 on M9 minimal medium or M9 minimal medium supplemented with 10% ADS, respectively; cultures were spotted (5 μL) onto the indicated medium.
Strains were grown at 30°C with rich medium (YE5S) or minimal media (EMM) with essential supplements (Moreno et al., 1991).
For the analysis of sexual development, S. macrospora was grown on complete medium biomalt maize medium (BMM) (Esser 1982) or minimal medium Sordaria Westergaards medium (SWG) (Nowrousian et al. 2005).
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