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Every 45 yards of front had a medium or heavy howitzer for bombardment, which required 378 guns, with guns and howitzers (5%) deployed with the field artillery that was due to fire the creeping and standing barrages.
The population of cocaine users was divided into two groups; socially integrated individuals (experimental, recreational and heavy users) and marginalised individuals (light, medium or heavy users).
Each of the crop operations is designated as light, medium or heavy work and relevant tractor sizes are allocated to each work type.
Control cells (pBabe plasmid empty vector) were labeled light while Flag-His ISG15 ectopic expression cells (pBabe-Flag-His ISG15 mature) were labeled medium or heavy.
Briefly, cells were grown in the presence of light, medium, or heavy arginine and lysine followed by treatment with either PCI-34051 (10 μM), BRD3811 (10 μM), or vehicle (DMSO) for 24 h.
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U2OS cells transfected with pcDNA3.1, FLAG-Aven, or FLAG-AvenΔRGG were light (L), medium (M), or heavy (H) SILAC labeled, respectively, and IP with anti-FLAG antibodies.
Examples include squad leader, sniper, Ratelo (radio telephone operator), grenadier, scout, light rifleman, medium rifleman, or heavy gunner.
But about a quarter of our on-road petroleum is used in heavier diesel-powered commercial vehicles, such as buses and medium- or heavy-duty trucks.
We then performed quantitative proteomic analysis using an adapted approach combining stable isotope labeling with amino acids in cell culture (SILAC) and MS. Individual NIH3T3 fibroblasts populations were grown in defined SILAC medium containing light (no isotope label), medium (Lys4, Arg6) or heavy (Lys8, Arg10) amino acids (Supplementary Figure 1).
Human RPE1 cells expressing either GFP, GFP-VPS35, or GFPVPS35(D620N) were cultured in light DMEM (GFP), medium heavy (GFP-VPS35), or heavy SILAC medium (GFP-VPS35(D620N)), followed by lysis and precipitation with GFP Nanotrap beads.
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