Sentence examples for medium optimized for from inspiring English sources
After centrifugation, pellets were plated in culture flasks in the presence of 20 ng/ml human recombinant EGF and bFGF in basal serum-free medium optimized for neural stem cell growth [6], [36].
Pellets were dissociated in a 200 µl pipette tip, plated in an untreated 25 cm2 tissue culture flasks and cultured in medium optimized for NCs growth in neurospheres [50] Neurospheres were harvested and dissociated at single cells with collagenase I and re-plated under the conditions described above.
We found that when hES cell derived hepatic endoderm cells were plated on mitomycin-treated murine embryonic stromal feeders (STO) and cultured in hepatic progenitor expansion medium —a serum-free medium optimized for the proliferation of progenitor cells from rat hepatocytes [18] parenchymal cell colony appeared (Figure 3A and B).
N2 is a serum-free medium optimized for roNSC cultures, thus our initial hNSC culture setup utilized N2.
The results obtained in the growth medium optimized for VHG fermentations are summarized in Table 2 and in Figure 2A.
Based on the results of studies in a defined medium optimized for S. stipitis (ODM), the mechanisms of improved hydrolyzate performance varied among strains.
WIT Medium is a serum-free defined medium originally optimized for the robust culture of human primary mammary epithelial cells without the need of feeder cells57.
The following defined medium composition optimized for ethanol production from high xylose concentration feeds [ 10] was used in all precultures and growth cultures for inhibitor tolerance bioassays: purines/pyrimidines - 10 mg/L adenine, cytosine, guanine, uracil, and thymine; macro-minerals - 1 g/L K2HPO4, 1 g/L KH2PO4, and 0.75 g/L MgSO4.
M9-based culture medium was subsequently optimized for the enzyme production by orthogonal experimental design in shake flasks.
Culture conditions (i.e. seeding density, medium, serum supplementation) were optimized for long-term observation.
The reaction condition, poly phosphoric acid) (PPA)/phosphorous pentoxide (P2O5) medium at 130 °C, was previously optimized for the "direct" functionalization of carbon-based nanomaterials such as carbon nanotubes and carbon nanofibers [12 20].
