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This paper describes medium optimization for urease production by Aspergillus niger PTCC5011 by one-factor-at-a-time and orthogonal array design methods.
The medium optimization for the production of the Geobacillus thermoleovorans CCR11 thermoalkalophilic lipase was carried out in shake flask cultures using safflower high oleic oil.
Thus, medium optimization for VHG ethanol fermentation was found to trigger the increased yeast trehalose accumulation and plasma membrane ATPase activity.
This paper aimed to deeply analyze the mechanical, structural and thermal properties of solid-state medium, explored relationships between the medium's physical properties and fermentation performance, then to guide the process control and culture medium optimization for SSF.
Since medium optimization using a one-factor-at-a-time approach is time-consuming, expensive, and often leads to misinterpretation of results, statistical experimental design has been applied to medium optimization for growth and metabolite production [2 5].
Therefore, the fermentation medium optimization for simultaneous saccharification and fermentation (SSF) by using co-culture of Aspergillus niger and Saccharomyces cerevisiae for potato wastes was undertaken in this study.
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The approach for medium optimization adopted for improving AMVL production was divided into the following 3 steps: Because secondary metabolite production in filamentous fungi is strongly influenced by the carbon and nitrogen sources, the screening of carbon and nitrogen sources is crucial.
To our best knowledge, this is the first report illustrating a statistical medium optimization process for increased AMVL production in A. oryzae.
Factorial designs and second order response surface methodology (RS M for medium optimization were employed for the growth of recombinant Escherichia coli cells carrying a plasmid encoding TaqI endonuclease as a part of the fermentation strategy for general recombinant protein production.
Plackett Burman screening, factorial designs, and second-order response surface methodology (RSM) for medium optimization were employed for lovastatin production by a high-producing mutant of Aspergillus terreus.
For medium optimization, sucrose, yeast extract, sodium citrate, sodium acetate, NH4H2PO4, MgSO4, and MnSO4 were chosen for further optimization based on our preliminary experiments.
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