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The impact of different nitrogen sources and initial fermentation pH on enzyme production was studied in medium optimisation experiments using fractional factorial design.
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During the enzyme optimisation experiments, the concentration of H2O2 was kept constant.
The pH optimisation experiments for decolourisation showed that pH 4.0 and 3.0 were optimum for the decolourisation of MO and BB, respectively.
A series of optimisation experiments (see Additional file 1) showed that increasing temperature to 37 °C did not improve radiochemical yield at 5 min.
Therefore, in the future, it would be useful to include the murine IgG1 signal peptide in expression optimisation experiments for antibodies, which could improve yield.
In all of our DR optimisation experiments we have used our outbred laboratory strain of Drosophila, Dahomey.
In these optimisation experiments, primer dimers were not observed.
In initial optimisation experiments we investigated the effects of primer concentration on crossing point (Cp) determination.
Following initial optimisation experiments 500 ng of plasmid was used to transfect 2 × 10 cells in 24-well plates.
For this a series of optimisation experiments were performed on brain tissue samples (no. = 4 ME7 terminal 4 NB).
While we did not see primer dimers, non-specific amplification was occasionally seen during the optimisation experiments.
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