Sentence examples for medium onto a from inspiring English sources

In addition, to explore their endothelial potential, we re-cultured the sorted HEs in endothelial growth medium onto a Matrigel-coated plate.

In brief, exponentially growing monolayers were trypsinised and 10 cells were seeded in regular growth medium onto a 1% agar-coated well of a 24-well plate.

To assess anchorage-independent growth, 4.2 × 10 cells per cm were plated in 0.3% Noble agar (Sigma-Aldrich) in medium onto a base of 0.6% agar.

150,000 cells per well were plated in 2.5 ml of culture medium onto a 6-well plate 24 hours before the siRNA transfections.

Cells were layered in a medium onto a discontinuous Biocoll gradient (Biochrome, Berlin; two phases: 75 or 100% in PBS) and centrifuged for 30 min at 400  g.

For prostasphere-forming assay, 500 viable SP or non-SP PC3M-LN4 cells were suspended in serum-free medium onto a six-well ultra-low attachment plate (Corning, Invitrogen, Inc., Denver, CO, USA).

The multivalent molecular recognition between the Ad motifs on Ad-SiNWS and the β-cyclodextrin (CD) motifs on DNA⊂SNPs leads to dynamic assembly and local enrichment of DNA⊂SNPs from the surrounding medium onto Ad-SiNWS.

Here, hemocytes were perfused with 10 μL of Schneider's medium onto an etched ring containing a taxol solution that was instantly diluted to a final concentration of 1 μM.

We expressed NGL3 constructs similarly, but after two-fold dilution in PBS pH 7.5, we loaded cell medium directly onto a Ni affinity column.

Human PBMCs were isolated by the Ficoll gradient centrifugation, diluted 1 : 1 with RPMI-1640 medium, layered onto an equivalent volume of medium LSM-1077, and centrifuged at 700 g for 20 min at 22°C, as previously reported [ 5].

To maintain cell viability, overlay additional 1 ml 0.4% agar in culture medium onto each well once a week or when medium start turning yellow.