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In order to ensure that our flow cytometry results were reliable in the detection of apoptotic events we evaluated the expression of caspase-3 in cells treated with complete medium only, as well as complete medium plus IL-1ß + Fas.
The LPS showed significantly to stimulate the nitric oxide productions (P < 0.05) compared to the medium only (as the control), and this effect was abolished in the presence of polymyxin B (Fig. 2b) and showed no significant difference (P > 0.05) compared to the medium only (the control).
In addition, cells were added to 6 wells containing medium only as a negative control, and 2 wells containing phytohemagglutinin (PHA) (1 µg/ml in the well) as a positive control.
Seven participants used unstimulated PBMC (medium only) as a negative control sample.
We also incubated radiolabelled corticosterone without any tissue material in medium only as control incubations, to assess a possible spontaneous degradation of corticosterone.
Three wells were inoculated with 100 μl of fresh inoculum as for MABA and three more wells were incubated with 200 μl of culture medium only, as negative controls.
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Any medium is only as good as the people using it their skills make it good or bad.
Native, non-encapsulated VEGF at 10 or 20 ng/mL was used as the 100% bioactivity benchmark, and wells with medium only (no VEGF), as well as the released EGM-2 medium supernatant from non-loaded NPs, were employed as the negative control.
Please explain why in Figure 6D and 6E IFN- treatment was taken as negative control and not medium only treatment as shown in Figures A-C.
Three columns without drugs served as controls and one column with medium only served as blank.
In each plate, two columns without drugs served as controls and one column with medium only served as blank.
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Justyna Jupowicz-Kozak
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