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On this medium, only a very low percentage of the clones could grow, suggesting that mutations might have occurred either in the GLUT5 sequence or in the genome of the hxt 0 strain, as previously observed with GLUT1 and GLUT432.
Although a wide variety of yeasts are found to be present in the fermentation medium, only a few are able to compete with the selected strains of Saccharomyces cerevisiae that are known to dominate and persist in the fermentative environment.
The holdings are displayed in the Pavillon de Flore; due to the fragility of the paper medium, only a portion are displayed at one time.
After 7 10 days of culturing in this medium, only a few colonies remained; these were pooled together and propagated in the presence of selection-level puromycin.
After insulin-transferrin-selenium (ITS) was added to the same medium only a single Grp94−/− ESC line was established among 10 ESC lines derived from 24 outgrowths from 30 blastocysts.
Upon shifting these cells to starvation medium, only a minor fraction of Pex11 GFP is broken down, showing that this allele is strongly defective in pexophagy.
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The reality is that a medium only has a worth if it is prepared to take responsibility for its actions.
Low temperature (5 °C) and reduced aw (0.900 aw) during sporulation significantly reduced conidial germination times whereas the pH of the sporulation medium only had a slight effect at the tested values (2.5, 8.0).
Although the medium only became a nominee for the first time last year, the art form had already been accepted in the art world for several years.
Splenocytes were cultured at 37°C in flat bottom 12 well plates at a concentration of 1×107 cells/well and stimulated with 100 ng IRBP peptide or medium only in a volume of 0.5 ml.
In addition, cells were added to 6 wells containing medium only as a negative control, and 2 wells containing phytohemagglutinin (PHA) (1 µg/ml in the well) as a positive control.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com