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Addition of flg22 (50 μl of 22 μM flg22 per well) to the medium of two-week old seedling was for 2 hours.
DA (Sigma) was freshly prepared as a 100× solution in a Neurobasal medium and directly added to the culture medium of 4 week in vitro cultures, at least 5 days after changing half the medium.
Blasticidin (from 2 to 5 μg/ml of culture medium during 2 weeks) was used for the selection of clone of GL26 cells expressing EGFP.
Add 200 mL cold distilled water Weekly replace 50% of culture medium At week 6 and 12, take 10 of the non-adherent cells for analyses in clonogenic assays for CFU-GM.
Seedlings from 2 mg of 35S::AtAPY1-GFP transeedsc seeds were grown in 60 mL of liquid medium for two weeks and then ground in liquid nitrogen.
The maximum percentage seed germination (93.58 ± 0.56) was obtained in MS basal medium after 8 9 weeks of culture.
For the clonogenicity analysis, 24 h after transfection, aliquots of 500 viable HepG2-hbx cells or 1000 viable HepG2.2.15 cells were placed in 6-cm dishes and maintained in 10 ml of complete medium for 2 3 weeks.
The result showed that A. longa SW024T could grow on 1 20 dilution of MA plate, but only in 1 2 dilution of MB liquid medium, after two weeks' culture.
We also compared the DF stem cells cultivated in standard stem cell medium for 3 weeks in terms of osteopontin and alkaline phosphatase expression.
We present an analysis of medium-term (weeks to months) exposure effects of particulate pollution and temperature.
Selection was realized by adding 500 μg/ml of Geneticin selective antibiotic in complete medium of cell culture for 3 weeks.
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