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CD138-sorted cells were cultured either in the control medium (medium of untreated cells) or in the conditioned medium.
No mature cathepsin D bands were detected in the medium of untreated cells, or cells treated with EGTA (Fig. 3A).
Compared to that, the pH of the medium of untreated normoxic cells decreased to acidic pH values between 6.0 and 7.0.
Conditioned medium of untreated CT-26 cells was loaded into a single wide lane on gelatin-containing gel, electrophoresed, and the gel was divided into four strips.
In the CILAIR experiment we identified 192 proteins with incorporated label in medium of LPS treated tissue and 209 in medium of untreated adipose tissue.
In contrast, the exosomes purified from the medium of untreated MIN6B1 cells did not affect the survival of recipient cells (Additional file 4: Figure S3A).
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An equal volume of EtOH was added to the medium of the untreated control cells.
Infections were performed for each drug treatment by adding wild-type and mutant virus at an MOI of 0,5 in 100 µl of medium to untreated or treated cells.
Release of ZA was confirmed by extracting medium from non-chelated and chelated cell culture models with DDs and applying this medium to untreated fresh cell cultures, providing appropriate controls.
The development of chemoresistance was not observed in SMMC7721 cells when they were cultured with conditioned medium from the culture of untreated MSCs or from the culture of MSCs pretreated with either IFN-γ or TNF-α alone.
RD/18 clone cells (derived from the embryonal rhabdomyosarcoma cell line RD) treated with 100 nM dexamethasone showed an almost complete block of differentiation: about 5% myosin-positive cells were observed after 2 weeks of culture in dexamethasone-supplemented differentiation medium, compared to 20% of untreated cultures.
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