Sentence examples for medium of transfected from inspiring English sources
IL-12 p40 was first detected in the medium of transfected cells 12 hours after transfection.
(D) Representative immunofluorescence images of C2C12 cells cultured four days in a low serum medium of transfected 10T1/2 cells, as described in B. Differentiating myoblasts and myotubes were marked by the MF20 antibody (green).
Metabolically labeled proteins were precipitated from the conditioned medium of transfected 293 T cells with VEGFR-3-Ig fusion proteins, with antiserum 882 or with anti-V5-antibody (VEGF-C-CT is V5-tagged) and analyzed by 12% SDS-PAGE under both reducing and non-reducing conditions.
We previously produced a recombinant protein of human sAPPα 695 fused to the Fc domain of human immunoglobulin in the conditioned medium of transfected Cos-7 cells [5].
The medium of transfected cells was replaced by MEM with 10% FCS and 1% P/S at 6 hours post-transfection.
All the trimerbodies were isolated in functional active form from conditioned medium of transfected HEK293 cells and easily purified using immobilized metal affinity chromatography.
The evaluation of lactate dehydrogenase (LDH) activity in the culture medium of transiently transfected cultures revealed that the cytotoxicity depended on the transfection procedure, and not on the Myc-PARG over-expression (Figure S1 B).
Compounds of interest were added to the culture medium of cells transfected with both GFP-CL1 and α-synuclein 2 h after transfection.
Adding tunicamycin to the culture medium of cells transfected with GM-CSF del) also yielded a sinGM-CSF delglycosylalso species of about 18 kDa, but secretion was at a significantlyielded level than either the 29-kDa hyperglycosingled GM-CSF(del) proteinon-N-glycosylatedycin-treated cellspeciese 18-kDa nof-N-glycosylaboutfull-length GM-CSF from tunicamycin-treated cells.
Moreover, no virus with NL activity was produced from culture medium of cells transfected with pUC1.2xHBV/NL and pUC1.2xHBV‐D/MHD, both of which lack pol activity (data not shown).
Growth medium of stably transfected HeLa-CTL, HeLa-Cx37, HeLa-Cx40 and HeLa-Cx43 was supplemented with puromycin (1 μg/ml; Sigma Aldrich) and of HeLa-Cx43NT-GFP and HeLa-Cx43CT-GFP with zeocin (200 μg/ml; Invitrogen, Life Technologies).
