Exact(1)
The medium of the samples was aspirated out and cells were washed twice with PBS, whereafter 1 ml of neutral red medium (40 μ g/ml) was added to each well of the plate.
Similar(59)
In this stage, the external solution is diluted to 0.125 M of NaCl which generates an imbalance between the inner and outer medium of the sample.
The desorption peak of the medium acid sites of the samples centered in the range between 250 and 350 °C, and the desorption peak at 350 550 °C corresponded to the strong acid sites of the catalysts (Ma et al. 2007).
The medium compression resistance of the samples, cured in air conditions during 3 days is 4.0 MPa, on the 60th day arrived to 13.4 MPa and to 17.1 MPa on the 365th day.
The cells were then washed twice with 100 μl of FMF medium and then resuspended in 300 μl of FMF medium keeping the samples in the dark as much as possible.
To inhibit reminiscent copper toxicity on bacteria, a neutralizing solution (1.0 g sodium thioglycolate, 1.46 g sodium thiosulfate, 1000 ml deionized water), as described by Tilton and Rosenberg (Heidenau et al. 2005; Tilton and Rosenberg 1978), was added to the growth medium of all samples after the incubation period.
After treatment, cells were held in the treated medium for one minute (unless indicated otherwise) and then the cover slip was placed in a new 6-well plate containing 2 ml of complete medium, and the samples were returned to the incubator for one hour before analysis by immunofluorescence or western blot.
The SIA system was designed to add reagents to the reactor and to perform on-line sampling of the reaction medium, sending the samples to a flow-through spectrophotometer for monitoring the color reduction of the dye.
The culture medium without the extracts of the samples was considered as the control.
Cell-free DNA (cf-DNA) concentration was determined in the conditioned medium of aortic samples and in plasma, in both humans and in the experimental AAA model in rats, using Quant-it™ Picogreen® ds DNA Reagent (Invitrogen).
Levels of NO detected in the incubation medium of ICB samples increased from 1.49 ± 0.19 (control) to 8.81 ± 2.20 μM/mg protein (morphine-treated; 100 μM).
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