The medium of the samples was aspirated out and cells were washed twice with PBS, whereafter 1 ml of neutral red medium (40 μ g/ml) was added to each well of the plate.
The shape of the PSF for a specific bright-field microscopy setup is dependent on the NA of the objective used, the refractive index of the medium between the sample and the objective lens and the wavelength, λ, of the image forming light 6.
2. For parallel-beam absorption X-ray tomography under pressure, the presence of a pressure medium surrounding the sample presents a challenge for high-resolution imaging.
Note, however, that voxel intensities may be skewed under high pressure by the presence of the pressure medium surrounding the sample, especially after applying non-conventional flat-field normalization.
The glucose concentration in the medium of each sample was measured by using glucose assay kit (Biovision, Milpitas, CA, USA).
The size of the spheres also allows one to retract the sensor from the medium without a contamination of the sample.
The amount of sDsg2 released into the conditioned medium of each sample was quantified using Quantity One image analysis software.
Digitonin was then diluted with 10 volumes of medium A and the sample was centrifuged at 12 000 g for 10 min at 4°C.
To inhibit reminiscent copper toxicity on bacteria, a neutralizing solution (1.0 g sodium thioglycolate, 1.46 g sodium thiosulfate, 1000 ml deionized water), as described by Tilton and Rosenberg (Heidenau et al. 2005; Tilton and Rosenberg 1978), was added to the growth medium of all samples after the incubation period.
The desorption peak of the medium acid sites of the samples centered in the range between 250 and 350 °C, and the desorption peak at 350 550 °C corresponded to the strong acid sites of the catalysts (Ma et al. 2007).
