On the methodological side, traditional reconstruction techniques using the FBA framework needed in-depth adaptations: the fundamental ingredients, namely the specification of the medium and the objective function, are indeed unknown in this particular setting.
Additionally, in a traditional instrument care must be taken when preparing a sample to ensure that there is an appropriate amount of immersion medium between the objective and the coverslip, that no bubbles are present in the fluid, and that the objective is focused at an appropriate depth above the glass.
In this paper, we investigate the optimal configuration of a porous medium structure with the objective to minimize the hot spot temperature in natural convection.
The 3D OTF support, O s (k, k z ), for a high numerical aperture (NA) incoherent imaging system with a circular pupil is given by [ 9] O s (k, k z ) = [ (| k | − k 0 sin α ) 2 + (| k z | + k 0 cos α ) 2 ≤ k 0 2 ], (6 where N A = m sin α, m being equal to the refractive index of the objective immersion medium, and k 0 = 2 π m / λ 0, where λ 0 is the wavelength of the emitted light.
Moreover, the spherical aberration imposed on the focusing when passing from the immersion medium of an immersion objective lens (typically oil with n = 1.5) into diamond causes lateral and substantial axial enlargements of the focal diffraction maximum in proportion to the penetration depth, reducing the intensity further.
The preliminary FBA step in GIMME is naive about the in vivo medium composition and uses everything provided and suitable for the maximization of the objective function.
Include the medium of the publication.
Specify the medium of the article.
Accountability and transparency is in all work, especially in emergencies, fundamental to the achievement of the objectives of UNICEF's Medium-Term Strategic Plan 2006-2013 and the Core Commitments for Children (CCC), and underpins UNICEF's approach to results-based management and the Monitoring Results for Equity System (MoRES).
None of the objectives have been met.
