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Furthermore, signal peptides StII, LMSEA, LSEA-mut and XCs can secrete Chi92 to the extracellular medium of recombinant E. coli.
We have established a liquid chromatography mass spectrometry based metabolomics platform to identify extracellular metabolites in the medium of recombinant Chinese hamster ovary (CHO) fed-batch reactor cultures.
PDC accumulated in the medium of recombinant E. coli cells that had been transformed with genes isolated from Emericella niger, E. coli, Pseudomonas putida, and Sphingobium sp. SYK-6.
The result indicates that the oxalate present in the medium of recombinant strain was degraded as a consequence of secreted OxdC.
The results showed reduction in oxalate level in the medium of recombinant L. plantarum, while the medium of wild type L. plantarum showed no reduction in oxalate level.
Rice α-amylase was purified from the culture medium of recombinant Y. lipolytica by ammonium sulfate precipitation at 75%% saturation and subsequently subjected to β-cyclodextrin affinity chromatography, resulting in 72-fold purification at 47 % yield (Park et al. 1997).
Similar(53)
Cells were incubated for 7 days in mammosphere medium (DMEM: F12 medium supplemented with B27 without vitamin A [diluted 1: 50; Gibco]) and mammary epithelial growth medium aliquot of recombinant human epidermal growth factor (EGF) and gentamicin/amphotericin-B, (SingleQuot) (Lorne Laboratories).
For comparison, ST77 and 764 are classified as lines expressing relatively "high" and "medium" levels of recombinant protein as hTG accumulates to 1.61 % TSP [ 5] while mSEB protein accumulates to 0.76 % TSP in the 764 line [ 11].
The ELISA results for the surface protein in the medium of the recombinant MVA, strongly indicate that the recombinant virus has been successfully obtained.
The addition of several kinds of fatty acid to the culture medium of a recombinant human serum albumin (rHSA -producing yeast, Pichia pastorHSA -producingn increased expression levels of the product.
Colonies were isolated and they were grown in liquid medium for purification of recombinant plasmids on silica gel columns (Plasmid Mini, A&A Biotechnology, Poland).
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