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In good agreement with these findings we observed that when HMVEC-L were exposed to conditioned medium of primary alveolar epithelial cells, the increase in endothelial barrier function was more pronounced.
Using mass spectroscopy, mutant FN was detected in plasma and in culture medium of primary dermal fibroblasts isolated from the patient, but mutant protein was much less abundant than wild-type FN.
Exosomes isolated from the medium of primary cortical neuron cultures contain abundant microRNAs and small RNAs such as miR-124a.
Plaque assays revealed replication-competent progeny virus in the culture medium of primary human fibroblasts; 12,000 to 16,000 PFU/mL were detected at 24 hours post infection (Figure S3).
While an increase in protection was observed for all CysC concentrations added to the culture medium of primary cortical neurons, a decrease in protection by high CysC concentrations was observed for N2a cells.
TGFβ levels were assessed in 48-hour conditioned medium of primary lung fibroblasts that were cultured in DMEM (2% FCS, 1% penicillin-streptomycin, 10% BSA).
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Although a conditioned medium of primary-cultured astrocytes can be effective in culturing neurons for a longer period of time, the use of primary astrocytes may not be practical due to a number of limitations, including restricted availability of neural tissues as source of astrocytes, and extensive time and effort to obtain astrocytes from living tissues.
It cost $853-$853-$1489edium level of primary care and $2581- $4252 of high level of primary care to save one YLL.
Across five PCDs, the cost of saving one hospitalisation was estimated to be in the range of $134-$134-$404he medium level of primary care, and $425-$425-$1168igh level primary care (Table 4).
In fact, hydrogen can operate as a storage and carrying medium of these primary sources.
Addition of conditioned medium of mouse primary ATI-like cells alone resulted in enhanced VE-Cadherin staining in the junctional zone.
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