Sentence examples for medium of parental from inspiring English sources

All G418-resistant (G418R) Mclonesones were grown in the same medium of parental MCF7 cells plus 0.5-1 mG418 G418 (Cellgro/MediaTech and Hyclone).

Experiments with conditioned medium of parental U87MG cells rescued the downregulation of IL6 and IL8 after Evi silencing (Fig S7A of Supporting information).

After 5 weeks of culture, the calli were transferred to fresh medium of parental composition for further multiplication and to obtain regeneration of plants from them.

Matrix metalloproteinase-2 and MMP-9 activity in the conditioned medium of parental NIH OVCAR3, Ctrl scFv and CA125/MUC16 knockdown cells were analysed using 10% SDS gelatin zymography.

A suspension of 300,000 cells/mL in serum-free medium of parental and IM3 cells transfected with LNA antagomirs (against miR-143 and miR-145 or a control LNA antagomir) was plated in the upper chamber of a Boyden manifold over a Matrigel-coated membrane (24-well insert; pore size, 8 um; BD Biosciences).

Cells stably expressing KV10.1 (HEK-KV10.1 and HEK-KV10.1-AP) were cultured in the medium of the respective parental cell lines supplemented with Zeocin (Calya, 3 µg/mL).

Once the Htop medium had solidified, 3 μl of serial dilutions of parental phage stocks were spotted onto the surface of the plates.

The L-lactate enriched medium from conditioned WI-38 cells, but not medium from parental fibroblasts, was able to significantly sustain the growth of both LNCaP and PC3 cells in presence of low glucose.

To then isolate haploid gene-replacement strains, merodiploids were subcultured absent Tc selection in rich GYPC liquid medium and then plated with Tc added at very low (0.125 µg/ml) levels sufficient to 50% inhibit growth of parental wild-type.

A lack of parental time and engagement is self-evidently a bad thing, as is the excessive use of any one medium.

And slowly, thoughts of parental failure recede.