Sentence examples for medium of differentiated from inspiring English sources

The medium of differentiated hypothalamic neurons culture was removed and cells were immediately frozen in dry ice.

It was found that treatment with 1 µM of CID755673 completely blocked the killing activity of the conditioned medium of differentiated HL-60 cells (Figure 4B).

Subsequently, cells were loaded with 5 mM fura-2-AM (Molecular Probes) and 0.025% pluronic-F127 (Molecular Probes), added to the medium of differentiated podocytes for 20 minutes at 37°C.

For infection, the medium of differentiated THP-1 and blood monocytes-derived macrophages was removed and replaced with 1 ml of bacterial suspension from each strain (Mtb MT103, its phoP mutant SO2, and its complemented strain SO2-pSO5) in RPMI containing 105 cfu/ml, to obtain a multiplicity of infection (MOI) of 1∶10, bacteria per macrophage.

Kinetic quantitative determination of alkaline phosphatase (ALP) was carried out in the medium of differentiated cells using a commercial kit provided by Stanbio Laboratory (Boerne, TX, USA).

(K ) Glycerol levels in medium of differentiated primary white adipocytes from Angptl4 -/-, wild-type and Angptl4 -Tg mice, serum-starved and treated with 10 μM isoproterenol for 3 hr.

After 15 days cultured in selection medium, majority of differentiated and precursor cells died due to short lifespans, e.g., granulocytes 30-400 minuthe in the peripheral blood with a total lifespan of 7-13 days that varied under certain pathological conditions) [ 7], monocytes (5-7 days) [ 8] and platelets (3-5 days) [ 9].

Since A2ML1 is a secreted protein [15], we next assessed whether p170 could be detected by immunoprecipitation with PNP sera from the culture medium of radiolabeled differentiated keratinocytes.

Removal of retinoic acid from the culture medium of fully differentiated cells gradually results in the development of a squamous phenotype (Yoon et al. 1997).

After an 8-day treatment with the mixed conditioned medium, the number of differentiated osteoclasts was determined using TRACP staining assay kit according to the manufacturer's instructions (Sigma).

Moreover, although submerged cells that have not undergone organotypic differentiation can be exposed directly to complex aerosols such as CS, 9– 12 the nonspecific stress and damage caused by removal of the culture medium and the lack of differentiated features that protect against airborne toxicants (eg, mucociliary or squamous differentiation) cannot be excluded.