Considering the measurements of Lewandowski et al. (2003), who determined cellular in vitro concentrations relative to corresponding medium Hg concentrations, this LOAEL is equivalent to an in vitro medium concentration of approximately 266 nM.
Figure 8b shows the temporal behavior of the emission current densities at different electric fields, which were measured at a medium vacuum of approximately 10−5 Torr.
No bars are shown for pex4-1 pex5-10 at 28 °C (D) because of the extremely poor germination rate (one seed germinated on 0.5 % sucrose-supplemented plant nutrient medium out of approximately 100 seeds plated; none germinated without sucrose or with 30 μM IBA).
The trematode Schistosoma mansoni Sm14 antigen was expressed in the yeast Pichia pastoris and secreted into the culture medium at yields of approximately 250 mg L−1.
Our results show that a correctly processed Sm14 was secreted into the culture medium at levels of approximately 250 mg L−1.
Overnight cultures of C. difficile grown in TY were used to inoculate fresh TY medium at OD600 of approximately 0.05.
Cells in NMF-conditioned medium had wounds of approximately 383 ± 38 μm at 0 hours, 213 ± 33 μm at 24 hours and 52 ± 16 μm at 48 hours, and cells cultured in CAF-conditioned medium had wounds of approximately 392 ± 52 μm at 0 hours, 58 ± 52 μm at 24 hours and 17 ± 29 μm at 48 hours.
A sample size of 150 (75 per group) was projected to have power of 1- β = .8, with significance level α = .05 and a medium effect size of approximately d = 0.4.
The 2D clean-TOCSY spectra (36) were acquired at short and medium mixing times of approximately 13 and 36 ms, respectively, with wrapping in the f1 dimension for high resolution in the sugar proton region.
Yeast was harvested from 50 ml of medium at OD600 of approximately 4 by centrifugation at 3500 × g for 4 minutes, and resuspended in 50 ml of 1.3% w/v sterile CaCl2 (Scharlau, Sentmenat, Spain) solution containing 1.3% w/v carboxymethylcellulose (Aldrich, Steinheim, Germany) with an average molecular weight of 250 kDa and degree of substitution of 0.9.
