Growth medium (negative control) without inoculation with yeast strains lacks detectable Hg aMass of EPS extracted from 20 ml yeast culture (OD600 nm = 1.2, approx).
For every experiment, a sterility check (10% aqueous DMSO and medium), negative control (10% aqueous DMSO, medium and inoculum) and positive control (10% aqueous DMSO, medium, inoculum and water-soluble antibiotics) were included.
The inhibitory activity of culture supernatants from B. amyloliquefaciens N2-4 and N3-8 isolates against B. pseudomallei by the agar well diffusion method as seen by clear zones, MM minimal medium (negative control), CAZ ceftazidime 50 µg/mL, the drug of choice for B. pseudomallei (positive control).
Then, the medium was replaced with a fresh medium (negative control) and the medium containing the PLGA/2 % CNTs, free DOX (positive control), PLGA/1.5 % DOX, and PLGA/1.5%DOX@2%2 % CNTs composite nanofibers, and the total DOX concentrations was designed 10 mg/mL, 25 mg/mL, and 50 mg/mL.
Subsequently, the medium was removed and the cells were exposed for 72 h with the sludge extracts water dilutions in L15 medium, negative control (L15 medium) and positive control using 100 to 3.125 pM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, Sigma-Aldrich, Deisenhofen, FRG).
The tested media included: [PAGS∶heparin∶FGF-2] release medium, negative control without FGF-2, positive controls of fresh FGF-2 and fresh [heparin∶FGF-2].
Flasks were supplemented with either 4 ml of conidial suspension or minimal medium (negative controls).
The cells were labeled with CD133/2-PE CD133/2-PE 10 min following the matufacturer's instructions, then washed and resuspended in stem cell growth medium; negative controls were performed with IgG2b(mouse)-PE antibody as recommended by the manufacturer.
The ATDC5 cells cultured on the PAMPS and PDMAAm gels in the maintenance medium were compared with the cells cultured on the polystyrene (PS) dish in the maintenance medium (the negative control) and in the differentiation medium (positive control).
For PHA and tetanus antigen, we used culture medium as negative control, and for adenovirus antigen, we used HFF culture supernatant as negative control (Institute of Virology, Ulm University Medical Center, Germany; final concentration 20 μl/ml).
Control wells were stimulated with concanavalin A at 5 µg/mL (positive control) or medium only (negative control).
