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It was determined that lactic acid (50%, w·w−1) was the main product of molasses and inulin hydrolysis in a supercritical water medium modified with basic catalysts.
The minimum inhibitory concentration (MIC) of Pb II) ions for SRC consortium was investigated using Postgate growth medium modified with varied concentration of heavy metal ranging from 10 to 110 mg/L.
In our previous work, we demonstrated that SH-SY5Y cells, cultured with a cell culture medium modified with PF-127 coated CNTs, are able to migrate under the effect of an external magnetic field [9] towards the magnetic source.
G361 cutaneous melanoma cells (ECACC, European Collection of Cell Cultures, Salisbury, UK) were grown in McCoy's 5a medium modified with 10 % FBS, 2 mM L-glutamine, and 1 % penicillin/streptomycin.
CP-A (ATCC® CRL-4027™ CRL-4027™oGFP-expressing CP-D cells (andC® CRL-4030™; transduced with MISSION® pLKO.1-puro-UbC-TurboGFP™) were maintained in serum-free Keratinocyte medium modified with 20 ng/mL epidermal growth factor, 140 μg/mL bovine pituitary extracells00 U/mL penicillin ATCC®00 μg/mL CRL-4030™ CRL-4030™).
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HT29 human colon cancer cells were maintained in McCoy's 5A medium (modified) supplemented with 10% fetal bovine serum.
As mineral medium, modified MBM with NaHCO3 as buffer, 5 mM ammonium and 1 mg/mL mannitol as carbon source (1 mL/L glycerol for R. denitrificans) was used.
The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
The human colon cancer cell lines HT-29 and SW-620 were propagated and cultured according to the distributor's protocol using McCoy's 5A medium modified and supplemented with 10% heat-inactivated FBS, streptomycin/penicillin (100 units/ml).
Human osteosarcoma Saos-2 cells (p53-null) (ATCC N°HTB-85) were grown at 37°C in 5% CO2 in Mc Coy's 5A medium modified (Invitrogen) supplemented with 15% fetal bovine serum and 1% penicillin-streptomycin-glutamine.
In further groups of animals, the cardiac GSH concentration was augmented by supplementing the perfusion medium, (modified Krebs Henseleit buffer (KHB), with NAC (4 mmol/L).
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