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Cells were exposed to medium mixed with urine (1 1), medium mix with PBS (Phosphate Buffered Saline) (1 1), only urine, and whole medium without cells as background.
Mycelia were harvested by vortexing in fresh, sterile PDB medium, mixed with sterile soil.
The cell number was significantly lower in all groups exposed on medium mixed with urine and urine alone.
Escherichia coli ER2566 expressing glycoside hydrolase was cultivated in a 2-l flask containing 450 ml of Luria Bertani medium mixed with 20 μg ml−1 of ampicillin for pTrc99a vector and kanamycin for the other vectors at 37 °C with shaking at 200 rpm.
Recombinant Escherichia coli ER2566 (New England Biolabs, Herfordshire, UK) expressing DT-bgl (GenBank Accession Number YP_002352162) or PF-bgl (GenBank Accession Number AAC25555) was cultivated at 37 °C in a 2000 ml flask containing 500 ml Luria Bertani medium mixed with 20 µg ml−1 kanamycin with rotation at 200 rpm in a shaker.
To prepare a paper-based medium, filter paper (Advantec 5B) was saturated with the basal medium mixed with selective solution, and air-dried.
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Cells were re-suspended in 50 μl serum-free growth medium, mixed 1 1 with Matrigel (Cat no. 354248; BD Bioscience) and injected in a total volume of 100 μl.
Reactions contained 50 PFUs of MERS-CoV (EMC/2012 strain) or BCoV (Nebraska strain) in 25 μL of medium mixed 1 1 with camel serum diluted in 25 μL serum-free Dulbecco minimum essential medium.
Cells were re-suspended in 50 ìl serum-free growth medium, mixed 1 1 with Matrigel (cat. No: 354248; BD Bioscience) and injected in a total volume of 100 μl, using a 27-gauge needle.
Mr. Canning uses Pro-Mix, a peat-based medium, mixed fifty-fifty with coarse sand.
This article of course is targeting only one specific scenario with a medium, mixed infantry-based force.
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