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1.4.3 Prepare the transfection mix by diluting 1 μg sgRNA in 50 µl serum free medium and 4 µl Fugene HD mix plus 46 μl serum free medium, mix both, and incubate for 45 min at room temperature.
Raise heat to medium, mix ingredients well and cover for about 30 seconds.
HUVECs were incubated in endothelial basal medium mixed with the same volume of 5-fold concentrated medium for cell proliferation, migration, and tube formation in vitro.
For an artist, whose day job involves honing the senses to their most refined capacities, love is like the medium mixed into all things.
Bump speed up to medium and mix for two minutes or until mixture is smooth.
2. In a medium mixing bowl, briefly break up the 454g cream cheese by beating it for 10 seconds with a hand mixer using low speed.
In a medium mixing bowl, combine the sugar, flour, cornstarch, and salt and mix well.
Get a medium mixing bowl.
Mix all ingredients in a medium mixing bowl.
Combine the marinade ingredients in the medium mixing bowl.
Transfer the fennel shavings to a medium mixing bowl.
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