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After attachment of the cells, wells were flooded with 0.5 mL of the same culture medium mix and experiments commenced.
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1.4.3 Prepare the transfection mix by diluting 1 μg sgRNA in 50 µl serum free medium and 4 µl Fugene HD mix plus 46 μl serum free medium, mix both, and incubate for 45 min at room temperature.
Raise heat to medium, mix ingredients well and cover for about 30 seconds.
Transfer this mixture to a medium mixing bowl and stir in the warm water, tahini, yoghurt, lemon juice and a pinch of salt.
In "To Draw a Line," metaphor and medium mix, as Ms. Antoni uses thread as the essential material with which to lure her audience into the web of her design.
2. After 20 minutes, pour the remaining wine into a medium mixing bowl and sprinkle the gelatin over it.
Place chocolate pieces in medium mixing bowl and set aside.
Put about half the jar of peanut butter into a medium mixing bowl and stir thoroughly.
Cuttings grown in rooting medium Peat mix and with growth regulator Dip & Root™ resulted in the lowest rooting and survival percentage.
Three days later, cells were placed in the same medium lacking MIX and dexamethasone.
Mix together sugar, flour,oats, cinnamon and nutmeg in a medium mixing bowl Mix in butter and peanut butter with pastry blender or fork.
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