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secretion in Schneider's medium, medium with ant brains and medium with mandibular muscles.
Incubation of the cells with just medium, medium with DTIC or zVAD-fmk served as controls.
Cells were either cultured in medium, medium with E64d/PepA, starvation medium (HBSS) or HBSS with E64d/PepA for 2 h.
Medium was removed and cells were washed in PBS; then PBS was removed and the cell pellet was quickly frozen at −2°C, ready for RNA-isolating or resolved in freezing medium (medium with 20% bovine serum and 10% DMSO (Sigma-Aldrich)).
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Human microvascular endothelial cells and immortalized murine embryonal fibroblasts (MEFs) were cultivated in DMEM (Dulbecco's modified Eagle's medium) medium supplemented with 10% FCS (fetal calf serum).
In experiments with conditioned medium, medium was replaced every day.
I've had softer rib eyes than the president steak ($16.50), ordered medium rare but received medium with a foil-wrapped, steamed, not baked, potato.
24 h later, the medium was switched to CDF12 medium with medium changes every day.
Figure 1 PHB content as percentage of cell dry weight (CDW) in R. eutropha cultures grown in (A) TSB (rich) medium, (B) PHB production medium (minimal medium with 0.05 w/v% NH 4 Cl and 2 w/v% fructose), and (C) PHB consumption medium (minimal medium with 0.1 w/v% NH 4 Cl and no exogenous carbon source).
The agar plate medium was the corresponding liquid medium with addition of 1.5% agar.
At this point we replaced the drug-free medium with medium containing 200 mM methyl sulfone.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com