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Nonetheless, as she drives around England searching for a trustworthy medium, life inevitably intrudes on Picardie's mourning.
The liver was excised and minced in William's E medium (Life technologies, A12176-01, GraNY Island, NY, USA).
Imaging was performed in Leibovitz L-15 phenol-free medium (Life technologies, Grand Island, NY) containing the same concentration of doxycycline and FBS.
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We also used a commercially available melanocyte medium, M254 Medium Life Technologiess).
Keratinocytes prepared from similar epidermal samples were cultured in KSF medium (Life Technologies) as described previously [21].
Cells were washed with PBS, and then coverslips were mounted on slides with ProLong Gold® anti-fade mounting medium (Life Technologies Ltd., Paisley, UK).
The cell lines were maintained in DMEM medium (Life Technologies, Rockville, MD), while cell lines RMA and RMA-S was cultured in RPMI 1640 medium.
HepG2, H7402, HepG2.2.15, MHCC-97L, MHCC-97H cells were maintained in Dulbecco's modified Eagle's medium (Life Technologies, Inc., Gaithersburg, MD) supplemented with 10% fetal bovine serum (Life Technologies).
Nutrient deprivation was performed in EBSS medium (Life Technologies).
Coverslips were applied with Dapi-containing medium (Life Sciences).
Liver digest medium (Life Technologies) was added during four consecutive 30-min incubations at 37°C.
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