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The medium lethal dose (LD50) was determined using the Behrens and Karber method [ 14].
The medium lethal dose of SM is 40 80 g/kg, which implies that SM has low acute toxicity compared to the toxicity of most of the other herbal medicines [ 16].
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Accordingly this extract is safe for up to a dose of [medium lethal dose (LD50) could be greater than] 2 g/kg body weight in rats.
The effective therapeutic dose was taken as one tenth of the median lethal dose (LD50 >2.0 g/kg) [13].
For mammalian, metazachlor acute toxicity is low via oral, dermal, and inhalation routes [LD50 >2000 mg/kg (median lethal dose) and LC50 >34.5 mg/L (median lethal concentration)].
The data of MLA indicated that the lethal toxicity were 1.3 × 107 median lethal dose (LD50)/mL for BoNT/A and 1 × 106 LD50/mL for BoNT/B (Table. 1).
Despite these paradoxic effects with the most lethal dose, lethality with the medium cell-wall dose was associated with progressive reductions in blood pressure and increases in heart rate, as would be expected in a state of sepsis.
lethal dose with 50% lethality.
LT50, 50% lethality time; WHO, World Health Organization; LD50, 50% lethal dose.
After 2 weeks of incubation, single-hybridoma clones were picked up from semisolid medium, transferred to 96-well tissue culture plates (Costar, Corning, NY, USA), and then grown for 1 week in Clonacell Medium E with 5 ng/mL ricin (20 × hybridoma cell culture lethal dose) for further selection.
The mean lethal dose (D0) for tumor cells treated with radiation plus T-oligo, control-oligo or medium alone was 1.46, 2.98 and 3.36 Gy, respectively.
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