Juvenile birds are known to possess poorer competitive skills when fighting to settle in medium-large colony sizes, thus becoming relegated to breed solitarily or in small colonies [18].
The assay is performed on 1 2 week-old soil extract plates typically bearing 15 50 medium-to-large colonies of different strains.
To test this hypothesis, we stably transfected the parental MSU-1.1 cell strain with vectors encoding GFP-Rac1V12 or GFP-Cdc42V12 proteins (Fig. 4a), and assayed them for the ability to grow in medium with reduced serum, form large colonies in agarose, and develop into sarcomas in athymic mice.
We compared frequencies of fights between pairs of fighters and between fighters and scramblers for large colonies, and for medium-size colonies pooled for all densities (as the frequencies did not differ significantly between densities, Yates χ = 0.079, df = 2, P = 0.961).
First, we observed that Raji cells, but not primary B cells, grow extensively and develop large colonies upon plating on CFU medium.
A single colony was then inoculated into each well (containing 500 µl of sterile medium) of an 8-welled slide, such that small and large colonies of all three genotypes were equally represented on every slide.
In both cases, fights between fighters occurred disproportionately more often (large colonies, χ = 7.125, df = 1, P = 0.007; medium-sized colonies, χ = 15.244, df = 1, P < 0.001) than expected (Table 2).
To reconfirm this observation, one of large colonies on SC-Ura, carrying the original pTLU2, was plated on a complete medium (SC) for single colonies.
Here & there are large colonies of squatters.
Bumblebees nest in the ground, sometimes in large colonies.
