Sentence examples for medium lacking phenol from inspiring English sources

The medium was exchanged with fresh medium lacking phenol red.

For negative controls, unstimulated neutrophils in regular RPMI medium lacking phenol red were used.

Briefly, 18-days old BMMCs were washed with PBS and resuspended in culture medium lacking phenol red.

After 6 h, the medium was replaced again with serum-free medium lacking phenol red and supplemented with 1% CS-FBS, treatments were added at this point and cells were incubated for an additional 18 h.

However, oestradiol interferes with the effects of trastuzumab on HER-2 signalling and phenol red acts as an oestradiol-like factor (Treeck et al, 2003); therefore, before any in vitro study, OVCAR-3 cells were cultured for a week in an otherwise identical medium lacking phenol red.

TEC were cultured on 24-well plates (Falcon Labware, Oxnard, CA, USA) at a concentration of 5 × 104 cells/well and incubated with different plasma concentrations and 250 μg/ml XTT (Sigma, St . Louis MO, USA) in a medium lacking phenol red.

For experimental purposes, the cells were maintained in the same culture medium but lacking phenol red and containing charcoal-stripped FBS.

Bacteria were grown in Luria Bertani broth (LB) or Dulbecco's modified Eagle medium (DMEM) lacking phenol red (Gibco-BRL), without antibiotics and bovine fetal serum at 37°C without shaking.

The EPEC E2348/69 strain was grown in LB broth overnight at 37°C without shaking, OD600 was taken to measure the number of bacteria, and then diluted 1∶100 into Dulbecco's modified Eagle medium (DMEM) lacking phenol red (Gibco-BRL) with 0.56 mg/ml of peptides into 12-well plates.

Complete medium was composed of IF-M1 medium (Biolog, US) lacking phenol red, glucose, and glutamine, supplemented with 3.75% FBS, 1.25% chicken serum (Sigma-Aldrich, Germany), 11 mM glucose, and 2 mM glutamine (Sigma-Aldrich, Germany).

Cell culture medium was exchanged to DMEM lacking phenol red and supplemented with sodium pyruvate and HEPES buffer on the day of data collection.